Table 3.
| A Summary of identified Bp putative immunogenic proteins using the mouse model (** — Table 2A). | ||||
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| T | C | D | F | |
| Total PIPs | 19 | 31 | 31 | 12 |
| Total PIPs with CR or NS with normal mouse IgG bound-beads | 6 | 7 | 12 | 4 |
| Total PIPS without CR or NS with normal IgG bound-beads | 13 | 24 | 19 | 8 |
| % of total surfaceome (EMF) with tentative antigenic protein potential | 11% | 18% | 18% | 7% |
| B Putative Bp immunogenic proteins identified among Bp strains assessed in the study. | |||||||
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| Protein | Accession # |
Size (KDa) |
Localization | T | C | D | F |
| Autotransporter (Vag8) | NP_880953 | 101 | OM/E | 11(6)* | 16(10)* | 5(3) | |
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| Chaperonin GroEL | NP_882014 | 60 | C | 12(4) | 14(5) | ||
| Elongation factor Ts | CAE33099 | 34 | C | 19(5) | |||
| Elongation factor Tu | NP_878925 | 44 | C | 8(2) | 15(4) | ||
| F0F1 ATP synthase subunit B^ | NP_881826 | 17 | CM | 10(1) | |||
| Fructose-1,6-bisphosphate aldolase | NP_880254 | 39 | C | 14(4) | |||
| Glyceraldehyde-3-phosphate dehydrogenase | NP_879794 | 37 | C | 8(2) | |||
| HP BB 4955 | NP_891489 | 74 | U | 6(2) | |||
| HP BP 0205 | NP_879093 | 21 | U | 10(2) | 22(2)* | ||
| HP BP 0455 | NP_879313 | 74 | U | 3(1) | |||
| HP BP 1057 | NP_879842 | 12 | U | 23(2) | |||
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| HP BP 1440^ | NP_880182 | 34 | U | 22(4)* | 12(2)* | 34(6)* | 8(1) |
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| HP BP 1485^ | NP_880222 | 58 | C | 3(1)* | 15(6)* | ||
| HP BP 2191 | NP_880839 | 48 | U | 7(2)* | 8(2) | ||
| ketol-acid reductoisomerase | NP_879606 | 37 | C | 13(3) | |||
| N-acetyl-gamma-glutamyl-phosphate reductase | NP_881539 | 39 | C | 16(4) | |||
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| Outer membrane porin protein OmpQ | NP_881933 | 40 | OM | 8(2) | 8(2)* | 13(3)* | 8(2) |
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| Outer membrane porin protein precursor | NP_879650 | 42 | OM | 30(7)* | 50(13)* | 35(9)* | 35(9)* |
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| Outer membrane protein A precursor | NP_879744 | 21 | OM | 17(4)* | 21(5)* | 17(4)* | 35(4)* |
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| Pertactin^ | BAF35031 | 101 | OM | 6(3) | 4(2) | 2(1) | |
| Phospho-2-dehydro-3-deoxyheptonate aldolase | NP_881490 | 39 | C | 13(3) | |||
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| Preprotein translocase subunit SecD | NP_879831 | 69 | CM | 5(2) | 7(3)* | 5(2) | |
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| Putative bifunctional protein | NP_882247 | 43 | CM | 8(2) | |||
| Putative binding-protein-dependent^ | NP_881859 | 42 | P | 10(2) | 12(3) | 3(1) | |
| Putative efflux system transmembrane…^ | NP_880739 | 118 | CM | 4(2) | 2(1) | ||
| Putative exported solute binding protein | NP_881542 | 40 | U | 8(2) | |||
| Putative inner membrane protein | NP_881862 | 26 | U | 15(2) | |||
| Putative inner membrane protein | NP_886531 | 62 | CM | 6(2) | |||
| Putative inner membrane-anchored protein | NP_880838 | 33 | U | 8(2) | 8(2) | ||
| Putative l-lactate dehydrogenase | NP_879251 | 39 | C | 11(3) | |||
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| Putative lipoprotein | NP_881568 | 18 | OM | 44(4) | 14(2) | 49(5)* | 35(3)* |
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| Putative lipoprotein | NP_880303 | 41 | U | 12(3) | |||
| Putative outer membrane ligand binding protein | NP_879893 | 144 | OM | 4(3) | |||
| Putative peptidase | NP_880436 | 32 | OM | 16(2) | |||
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| Putative peptidoglycan-associated lipoprotein^ | NP_881875 | 18 | OM | 17(2) | 29(3)* | 7(1) | |
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| Putative periplasmic solute-binding protein | NP_880224 | 40 | U | 15(4) | |||
| Putative secreted protein^ | NP_879832 | 13 | U | 21(1) | 32(2) | ||
| Putative sulfatase | NP_881701 | 72 | CM | 4(2) | |||
| Probable inner-membrane protein | CAE35357 | 61 | CM | 6(2) | |||
| Rod-shape determining protein | NP_879246 | 38 | C | 16(4) | |||
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| SCO1/SenC family protein^ | NP_882237 | 22 | U | 6(1) | 6(1) | 6(1) | |
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| Serotype 3 fimbrial subunit | NP_880302 | 22 | E | 17(2) | |||
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| Serum resistance protein (BrkA) | NP_882013 | 111 | OM | 4(3)* | 3(2) | 4(3) | 4(3) |
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| Signal peptidase I | NP_881060 | 32 | CM | 7(2) | |||
| Surface antigen | NP_880169 | 86 | OM | 6(3) | |||
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| Thiol:disulfide interchange protein | NP_882154 | 71 | CM | 3(2) | 3(2)* | 3(2) | |
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| TonB-dependent receptor for iron transport | NP_879666 | 82 | OM | 4(2) | 5(3) | 13(7) | |
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| Tracheal colonization factor precursor | NP_879974 | 71 | OM | 6(3)* | 7(3)* | 7(3)* | 9(4)* |
Abbreviations: T — Tohama I, C — C056, D — D946 and F — F656; CR — cross-reactivity, NS — non-specificity, IgG — immunoglobulin G, EMF — enriched membrane fraction, PIPs — putative immunogenic proteins.
Values noted represent percent amino acid coverage of the identified IP protein using the mouse model. (Number of unique peptides detected in parenthesis.). No numerical value noted indicates that the protein was not identified in the mouse IP.
indicates that identified proteins only had one unique detected tryptic peptide.
Asterisk (*) indicates that the protein identified in the mouse IP was cross-reactive with normal mouse IgG-bound beads. Blue-shaded box: common in all 3 clinical isolates; black-bordered box: common in all 4 strains.
Abbreviations: T — Tohama I, C — C056, D — D946 and F — F656; OM — outermembrane, CM — cytoplasmic membrane, C — cytoplasm, P — periplasm, E — extracellular, M — membrane, U — unknown, HP — hypothetical protein, BB — B. bronchiseptica.