Table 4.
| A Summary of identified Bp putative immunogenic proteins using the human model (** — Table 2A). | ||||
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| T | C | D | F | |
| Total PIPs | 4 | 12 | 8 | 10 |
| Total PIPs with CR or NS with normal mouse IgG bound-beads | 3 | 9 | 8 | 7 |
| Total PIPS without CR or NS with normal IgG bound-beads | 1 | 3 | 0 | 3 |
| % of total surfaceome (EMF) with tentative antigenic protein potential | 1% | 2% | 0% | 2% |
| B Putative Bp immunogenic proteins identified among Bp strains assessed in the study. | |||||
|---|---|---|---|---|---|
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| Protein | Accession # | T | C | D | F |
| Autotransporter (Vag8) | NP_880953 | 12(8)* | 17(12)* | 7(4)* | |
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| HP BP 0205^ | NP_879093 | 6(1)* | 21(4) | ||
| HP BP 1440 | NP_880182 | 10(3) | 27(5)* | 11(3) | |
| HP BP 1485 | NP_880222 | 9(3)* | 16(7)* | 6(2) | |
| HP BP 3689 | NP_882194 | 18(2) | |||
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| Outer membrane porin protein OmpQ^ | NP_881933 | 3(1)* | 7(2)* | 11(3)* | 4(1)* |
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| Outer membrane porin protein precursor | NP_879650 | 23(6)* | 21(7)* | ||
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| Outer membrane protein A precursor | NP_879744 | 10(3)* | 21(5)* | 21(5)* | 17(3)* |
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| Preprotein translocase subunit SecD | NP_879831 | 12(5)* | |||
| Probable inner membrane protein | CAE35357 | 4(2)* | |||
| Putative lipoprotein | NP_881568 | 37(3)* | 22(2)* | ||
| Serum resistance protein (BrkA) | NP_882013 | 2(2)* | |||
| Thiol:disulfide interchange protein | NP_882154 | 3(2) | |||
| TonB-dependent receptor for iron transport | NP_879666 | 7(4) | 19(11)* | 3(2) | |
| Tracheal colonization factor protein | NP_879974 | 9(4)* | 10(5)* | 7(3)* | |
Abbreviations: T — Tohama I, C — C056, D — D946 and F — F656; CR — cross-reactivity; NS — non-specificity; IgG — immunoglobulin G; EMF — enriched membrane fraction; PIPs — putative immunogenic protein.
Values noted represent percent amino acid coverage of the identified IP protein using the human model. (Number of unique peptides detected in parenthesis.). No numerical value noted indicates that the protein was not identified in the human IP.
indicates that identified proteins only had one unique detected tryptic peptide.
Asterisk (*) indicates that the protein identified in the human IP was cross-reactive with normal human IgG-bound beads. Pink-shaded box — protein identified in human and mouse IP; yellow-shaded box — strain-specific protein identification only in human IP; black-bordered box: common in all 4 strains.
Abbreviation: T — Tohama I, C — C056, D — D946 and F — F656.