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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Mol Microbiol. 2013 Oct 29;90(6):1201–1215. doi: 10.1111/mmi.12425

Fig. 5.

Fig. 5

Characterization of pilin protein membrane associations.

A. The cell wall, cytoplasmic and membrane fractions from wild-type (BD2528) cells were analysed by Western blotting with EF-G, ComEA, ComGA, ComGC and ComGG antisera.

B–E. Membranes isolated from cultures of (B, D) wild-type (BD2528) or (C, E) ComGD over-producing (BD5727) strains were incubated with buffers containing 0.75 M NaCl, 4 M urea, 8 M urea or 1% Triton X-100 as described in Experimental procedures. The soluble and insoluble fractions were separated by ultracentrifugation. Samples were immunoblotted with (B, D) ComGA, ComEA, ComGC, ComGG or (C, E) ComGD antisera.