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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Biochem Pharmacol. 2017 May 26;140:139–149. doi: 10.1016/j.bcp.2017.05.018

Fig. 1.

Fig. 1

In silico analyses of the interaction between hsa-miRNA-370-3p and CYP2D6 mRNA. (A) According to the TCGA public database, the level of hsa-miRNA-370-3p was negatively correlated with the CYP2D6 mRNA level in non-tumor human liver tissues (r = −0.279 P = 0.049). The data points represent the relative expression levels of CYP2D6 mRNA and hsa-mir-370-3p, and the unit was defined as RPKM (Reads Per Kilobase Million). (B) Predicted hybrid complexes formed by hsa-miR-370-3p and its targeted sequence within the coding region of wild-type CYP2D6 mRNA. The free energy of hybridization is −29.2 kcal/mol. (C) A mutated hsa-miR-370-3p sequence was designed to include three altered nucleotides (italic, underlined). The predicted free energy of hybridization of the mutated hsa-miR-370-3p sequence is −17.7 kcal/mol.