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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Biochem Pharmacol. 2017 May 26;140:139–149. doi: 10.1016/j.bcp.2017.05.018

Fig. 5.

Fig. 5

Hsa-miR-370-3p inhibited drug-induced CYP2D6 expression in HepG2 and Huh7 cells. (A) Cells were seeded in 24-wells plates and dexamethasone was added to the DMEM medium at final concentrations of 1 μM, 2 μM, or 4 μM for a period of 72 h. After dexamethasone treatment, the CYP2D6 expression in HepG2 and Huh7 cells were upregulated in both mRNA and protein levels. The enzyme activity was also increased in a concentration-dependent pattern. (B) MiRNAs transfection of HepG2 and Huh7 cells prior to dexamethasone treatment showed that the induction of CYP2D6 by dexamethasone could be attenuated by hsa-miR-370-3p mimic, whereas hsa-miR-370-3p inhibitor increased the CYP2D6 levels. Data shown as mean ± SD (n = 3). *P < 0.05 vs. Negative Control.