Table 1.
RT Primers & Oligonucleotides | Sequences | Application |
---|---|---|
CYP2D6 | RT-F: 5′-TAGTGGTGGCTGACCTGTTCTCT-3′ RT-R: 5′-TCGTCGATCTCCTGTTGGACA-3′ |
RT-PCR |
GAPDH | RT-F: 5′-GAAATCCCATCACCATCTTCCAGG-3′ RT-R: 5′-GAGCCCCAGCCTTCTCCATG-3′ |
RT-PCR |
hsa-miR-370-3p | RT-F: 5′-GCCTGCTGGGGTGGAACCTGGT-3′ | RT-PCR |
U6 | RT-F: 5′-CTCGCTTCGGCAGCACA-3′ RT-R: 5′-AACGCTTCACGAATTTGCGT-3′ |
RT-PCR |
dye-miR-370-3p | /5IRD800CWN/rGrC rCrUrG rCrUrG rGrGrG rUrGrG rArArC rCrUrG rGrU | EMSA |
dye-miR-370-mut | /5IRD800CWN/rGrC rCrUrA rCrUrA rGrArG rUrGrG rArArC rCrUrG rGrU | EMSA |
cold-miR-370-3p | rGrCrC rUrGrC rUrGrG rGrGrU rGrGrA rArCrC rUrGrG rU | EMSA |
dye-miR370-Tar | /5Cy55/mCmU mUmCmC mUmGmC mCmUmU mUmCmU mCmAmG mCmAmG mGmC | EMSA |