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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Biochem Pharmacol. 2017 May 26;140:139–149. doi: 10.1016/j.bcp.2017.05.018

Table 1.

Primers used in qRT-PCR assays and oligonucleotides used in EMSA.

RT Primers & Oligonucleotides Sequences Application
CYP2D6 RT-F: 5′-TAGTGGTGGCTGACCTGTTCTCT-3′
RT-R: 5′-TCGTCGATCTCCTGTTGGACA-3′
RT-PCR
GAPDH RT-F: 5′-GAAATCCCATCACCATCTTCCAGG-3′
RT-R: 5′-GAGCCCCAGCCTTCTCCATG-3′
RT-PCR
hsa-miR-370-3p RT-F: 5′-GCCTGCTGGGGTGGAACCTGGT-3′ RT-PCR
U6 RT-F: 5′-CTCGCTTCGGCAGCACA-3′
RT-R: 5′-AACGCTTCACGAATTTGCGT-3′
RT-PCR
dye-miR-370-3p /5IRD800CWN/rGrC rCrUrG rCrUrG rGrGrG rUrGrG rArArC rCrUrG rGrU EMSA
dye-miR-370-mut /5IRD800CWN/rGrC rCrUrA rCrUrA rGrArG rUrGrG rArArC rCrUrG rGrU EMSA
cold-miR-370-3p rGrCrC rUrGrC rUrGrG rGrGrU rGrGrA rArCrC rUrGrG rU EMSA
dye-miR370-Tar /5Cy55/mCmU mUmCmC mUmGmC mCmUmU mUmCmU mCmAmG mCmAmG mGmC EMSA