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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Mol Cancer Res. 2017 Feb 23;15(6):651–659. doi: 10.1158/1541-7786.MCR-16-0466

Figure 2.

Figure 2

Depletion of CHIP enhances radiosensitivity independent of p53 status. A, A549 cells were treated with sh-NT or shCHIP-2 to stably deplete CHIP. Cells were treated with or without 10 Gy, harvested and immunoblotted for indicated proteins at indicated time. B, A549 cells (sh-NT or shCHIP-2) were treated with either control siRNA or p53 siRNA to deplete p53 for 48 h, irradiated (10Gy), and harvested after 24 h. Cell lysates were immunoblotted for indicated proteins. C, H1299 cells were treated with sh-NT or shCHIP-2 to stably deplete CHIP. Cells were treated with or without 10 Gy and harvested at indicated time points, Lysates were immunoblotted for CHIP and p21and tubulin as control. D, H1299 cells depleted of CHIP (shCHIP-1 and shCHIP-2) treated with different doses of IR. Immunoblot showing knockdown of CHIP (top) and clonogenic survival (bottom). *, P<0.05.