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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Mol Cancer Res. 2017 Feb 23;15(6):651–659. doi: 10.1158/1541-7786.MCR-16-0466

Figure 4.

Figure 4

CHIP interacts with p21 and promotes polyubiquitination and degradation in living cells. A, A549 cells stably expressing WT-CHIP were treated with 10 Gy, incubated for 24 h at which point MG132 was added for 4 h. Lysates were prepared and CHIP was immunoprecipitated and immunoblot analysis performed. B, Reciprocally, p21 was immunoprecipitated from lysate A and blotted for CHIP. C, A549 and D, H460 cells (sh-NT or sh-CHIP-2) were treated as A and ubiquitinated proteins were affinity purified using TUBE (see Methods and Materials) and immunoblotted for p21. 5% input shown in the bottom.