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CHIP promotes p21 ubiquitination in vitro. A, Purified recombinant CHIP was incubated with recombinant p21 (purified) in the indicated reaction mixtures at 37 °C for 1 h and immunoblotted for p21. B–D, CHIP and p21 were incubated as in A in the presence or absence of HSP70/HSP40 and immunoblotted for p21 (B), ubiquitin (C) and 48-linked ubiquitin (D).
