Figure 2. In vitro culture of FACS sorted CD56 and CD82 double negative (DN) and double positive (DP) human cells.

(A and B) On day 1 after sorting, both DN and DP cells appear bright and rounded, while some cells have flattened and firmly adhered to the plate. (C and D) On day 7 after sorting (4 days in growth medium and 3 days in differentiation medium [see Recipes]), DN cells remain mononuclear, whereas DP cells have fused and formed multinucleated myotubes (arrows). (E and F) Examples of immunostaining for myosin heavy chain (MHC) and desmin on double-positive cells are shown.