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. 2017 Sep 30;8(17):3555–3566. doi: 10.7150/jca.20952

Figure 3.

Figure 3

OXA-resistant cells (HCT116/OXA) exhibited up-regulated ZEB1 and an EMT phenotype. (A & B) Expression of epithelial biomarker (E-cadherin), mesenchymal biomarker (Vimentin) and matrix metalloproteinases (MMP2, MMP9) were measured by qRT-PCR and Western blot analyses. (C) Representative double immunofluorescence staining of the expression and co-localization of EMT biomarkers E-cadherin / Vimentin (Original magnification: ×400). (D) Transwell assays were used to determine the invasion capacity of HCT116/OXA and HCT116/OXA cells. Cells were starved for 12 hrs before being seeded into the matrigel chamber. (E) Scratch wound healing assays were used to test the migratory abilities of OXA-resistant cells and their parent colon cancer cells. (F & G) mRNA and protein expression of four EMT transcriptional factors (EMT-TFs): Snail, Slug, ZEB1 and Twist in normal colon epithelial cell and colon cancer cells. Compared to NCM460 group, #: P < 0.05, ##: P < 0.01, ###: P < 0.001. Compared to HCT116 group, *: P < 0.05, **: P < 0.01, ***: P < 0.001.