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. 2017 Sep 30;8(17):3555–3566. doi: 10.7150/jca.20952

Figure 4.

Figure 4

ZEB1 silencing promoted apoptosis and restored OXA-sensitivity of HCT116/OXA cells. (A & B) SiRNA1 candidate sequence pairs with the highest inhibition efficiency of ZEB1 expression confirmed by qRT-PCR and Western blot analyses were selected for subsequent experiments. The differences in ZEB1 expression levels among different groups were tested by one-way ANOVA analysis. (C) CCK8 assays were performed to identify the role(s) of ZEB1 in the OXA-sensitivity of colon cancer cells. (D) ZEB1 knockdown inhibited proliferation of HCT116/OXA cells, as measured by colony formation assays (P < 0.01). (E) The expression of apoptotic protein Caspase-3 and anti-apoptotic protein Bcl-2 measured by Western blot was normalized to GAPDH protein expression. (F) ZEB1 knockdown significantly enhanced OXA-induced apoptosis in HCT116/OXA cells (P < 0.05). All data represented as mean ± SD were from three independent experiments. *: P < 0.05, **: P < 0.01, ***: P < 0.001.