TABLE 2.

Summary of kinesin protofilament (PF) switching in the absence and presence of Tau and rigor kinesin obstacles

	Microtubule Obstacle	# Trajectories (avg length, μm)	Mean ± SD PF Switch Frequency (PF Switches / μm)	Total # Left PF Switches	Total # Right PF Switches	Median Left PF Switch Displace-ment (nm)	Median Right PF Switch Displace-ment (nm)
Kinesin-1	–	30 (1.5)	0.57 ± 0.74	18	18	30	32
	Low Tau	33 (1.4)	0.59 ± 0.74	16	20	21	23
	High Tau	31 (1.4)	0.74 ± 0.78	24	20	26	26
	Low RK	36 (1.8)	0.57 ± 0.64	24	21	34	30
	High RK	44 (1.8)	0.41 ± 0.60	21	22	28	33
Kinesin-2	–	31 (1.5)	2.20* ± 1.47	57	55	34	38
	Low Tau	30 (1.3)	2.08* ± 1.44	46	44	35	31
	High Tau	30 (1.6)	2.21* ± 1.28	53	52	40	38
	Low RK	30 (2.2)	2.19* ± 0.89	74	72	38	39
	High RK	32 (2.0)	2.48* ± 0.68	76	81	38	38

The mean (SD, standard deviation) protofilament (PF) switch frequency for each condition was calculated from a distribution made up of the PF switch frequencies of each trajectory. The median left/right protofilament switch displacement was calculated from a distribution made up of the left/right displacements for each experimental condition. A nonparametric ANOVA was applied to all distributions and a Dunn’s Multiple Comparisons Test was used to determine significance (p-value < 0.05) among groups.

^*Represents statistical difference from the kinesin-1 experimental condition.