Shape is summarized using principal component analysis of 17 landmarks. (A) PC2 separates benthic and limnetic males, with F2 females intermediate. In F2 females, PC2 is significantly associated with the male species chosen (B) and with variation in male PC2 scores when benthic males were chosen (C). In F2 females, centroid size is significantly associated with mate choice (D), but not with variation in male centroid size when benthic males were chosen or when limnetic males were chosen (E). See also Figure S1, Table S1, Table S3.