Key resources table.
Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact Yavin Shaham (Yshaham@intra.nida.nih.gov)
| Reagent | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit anti-c-Fos primary antibody | Cell Signaling Technology Phospho-c-Fos, 5348S, | AB_10557109 |
| Goat anti-CTb primary antibody | List Biological Laboratories CTb 703 | AB_10013220 |
| Mouse anti-mCherry primary antibody | Clontech Laboratories 632543 | AB_2307319 |
| Mouse anti-NeuN primary antibody | Millipore MAB377 | AB_2298772 |
| Biotinylated anti-rabbit IgG secondary antibody | Vector Laboratories | BA-1000 |
| Donkey anti-goat Alexa Fluor 488 | Jackson ImmunoResearch, 705-546-147 | AB_2340430 |
| Donkey anti-rabbit Alexa Fluor 594 | Jackson ImmunoResearch 711-585-152 | AB_2340621 |
| Biotinylated anti-mouse IgG secondary antibody | Vector Laboratories | BA-9200 |
| Critical Commercial Assays | ||
| Avidin-biotin-peroxidase complex ABC Elite kit | Vector Laboratories | PK-6100 |
| RNAscope Multiplex Fluorescent Reagent Kit | Advanced Cell Diagnostics | Cat# 320851 |
| Experimental Models: Organisms/Strains | ||
| Sprague-Dawley rats | Charles River | |
| Drugs | ||
| Methamphetamine | NIDA | |
| SCH39166 | Tocris Bioscience | Cat# 2299 |
| Raclopride | Sigma Aldrich | Cat# 98185-20-7 |
| Muscimol | Tocris Bioscience | Cat# 0289 |
| Baclofen | Tocris Bioscience | Cat# 0417 |
| CNO | Tocris Bioscience | Cat# 4936 |
| Tetrodotoxin (TTX) | Tocris Bioscience | Cat# 1078 |
| 4-Aminopyridine (4 AP) | Tocris Bioscience | Cat# 0940 |
| Retrograde traces and Viruses | ||
| CTb | List Biological Laboratories | Cat# 703 |
| AAV8-hSynp-hM4Di-mCherry | UNC vector core | Lot# AV5360D |
| AAV5-CaMKIIa-hChR2 (E123T/T159C)-EYFP | UNC vector core | Lot# AV4824 |
| Sequence-Based Reagents | ||
| RNAscope Probe Drd1a | Advanced Cell Diagnostics | Cat# 317031 |
| RNAscope Probe Drd2 | Advanced Cell Diagnostics | Cat# 315641-C2 |
| RNAscope Probe Fos | Advanced Cell Diagnostics | Cat# 403591-C3 |
| Software | ||
| iVision (4.0.15 and 4.5.0) | Biovision Technologies | |
| ImageJ | https://imagej.net/Fiji/Downloads | |
| MED-PC | Med-associates | |