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. 2017 Jun 20;7(6):630–637. doi: 10.1016/j.apsb.2017.05.006

Figure 2.

Fig. 2

CYZH was not an inhibitor of IAV envelope glycoprotein HA and IVA RNA polymerase (RdRp). (A) Inhibitory effects of CYZH on IAV RNA polymerase (RdRp) in MDCK cells. MDCK cells (2.5×105) were co-transfected with the plasmids expressing RdRp and then treated with CYZH and T705. RdRp activities were measured by Dual-Glo Luciferase Assay System. Con: treated with equal amounts of ddH2O as CYZH; CYZH: treated CYZH with the concentration of 2.5, 1.25 and 0.625 mg/mL, respectively; T705: treated with the concentration of 50 μmol/L.The experiments were performed in triplicate, and the data represents mean ± SD. ***P < 0.001 vs. Con. (B) The effect of CYZH on HA inhibition. The maximum concentrations (in row 1 of each plate) of CYZH, AH, OP and RBV were 2.5 mg/mL,10 μg/mL,10 μg/mL and 10 μg/mL, respectively. A serial of 2-fold dilution for all drugs were adopted in rows 2 and 3. The experiments were performed in triplicate.