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. 2017 Jun 20;7(6):630–637. doi: 10.1016/j.apsb.2017.05.006

Figure 3.

Fig. 3

CYZH up-regulated the expression of HO-1 mRNA and the protein. (A) MDCK cells were infected with IAV A/FM1/1947 (MOI = 0.005) in the presence or absence of 2.5 mg/mL CYZH for 6, 12 or 24 h. mRNA was collected in the indicated time points and then HO-1 mRNA expression was analyzed by qRT-PCR. (B) and (C) Infected MDCK cells were treated with or without CYZH (0.625, 1.25 or 2.5 mg/mL), AH (10 μg/mL), OP (10 μg/mL) or RBV (10 μg/mL) for 24 h. HO-1 mRNA was analyzed by qRT-PCR (B) and the protein by Western blot (C). The experiments were performed in triplicate, and each value represents mean ± SD. *P < 0.05, **P< 0.01, ***P < 0.001 vs. Mock; #P< 0.05, ##P < 0.01, ###P< 0.001 vs. IAV infected cells treated with equal concentration of solvent (Con).