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. 2017 Jul 27;12(8):715–723. doi: 10.1080/15592294.2017.1356959

Figure 4.

Figure 4.

Hypermethylation inhibits the activity of the KLF1 enhancer. (A) Analysis of KLF1 enhancer activity by a luciferase reporter assay in K562 cells. Luciferase activity was normalized to the activity of the pCpGL-CMV/EF1 Firefly control vector. Normalized activity of the construct containing unmethylated KLF1 enhancer CpG sites and the construct containing methylated KLF1 enhancer CpG sites are shown relative to the normalized activity of the reporter construct not containing the KLF1 enhancer sequence (empty vector). Error bars represent standard deviation of 3 transfection experiments. T test: ****P ≤ 0.0001. (B) Same experiment in non-haematopoietic HEK293T cells, confirming specificity of KLF1 enhancer function to erythropoietic cells.