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. 2017 May 30;12(8):637–652. doi: 10.1080/15592294.2017.1334025

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Figure 4. — Pyrosequencing replication of blood and hippocampal DMRs that fall on identical genes. (A) and (B) Bisulfite pyrosequencing for the DMRs in the Nav2 gene for the hippocampus and blood are shown, respectively. (C) and (D) Bisulfite pyrosequencing for the DMRs in the Slc38a2 gene for the hippocampus and blood are shown, respectively. (E) and (F) Bisulfite pyrosequencing for the DMRs in the Nr1d1 gene for the hippocampus and blood are shown, respectively. Note that DMRs for Nr1d1 in blood (gain of DNAm) and brain (loss of DNAm) are predicted to occur in opposite directions. Bar graphs in all of the panels are represented as mean ± SEM.*P < 0.05, **P < 0.01, and ***P < 0.001.