Table 2.
Serum bile acid profiles among groups
| Bile acids | RT_min | Parent ion (mz) | Product ion (mz) | Mean±SD
|
Fold Change (vs Con)
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Con | HFD | AB | SV | AB_SV | HFD | AB | SV | AB_SV | ||||
| TUDCA | 2.96 | 498.3 | 80 | 0.0805±0.0998 | 5.2828±6.9763 | 4.7295±5.0536* | 1.0151±0.4211 | 1.8002±0.9833& | 65.61 | 58.74 | 12.61 | 22.36 |
| GUDCA | 3.04 | 448.3 | 74 | 0.0017±0.0008 | 0.0046±0.0044 | 0.0042±0.0019* | 0.0029±0.0007 | 0.0043±0.0027 | 2.65 | 2.38 | 1.67 | 2.44 |
| THDCA | 3.07 | 498.3 | 80 | 0.1073±0.1149 | 0.182±0.1491 | 0.0886±0.0614 | 0.1136±0.1383 | 0.0673±0.0388 | 1.70 | 0.83 | 1.06 | 0.63 |
| TCA | 3.31 | 514.3 | 80 | 1.873±1.2593 | 0.5804±0.8325* | 0.2201±0.3018** | 0.1143±0.0843 | 0.2158±0.1544 | 0.31 | 0.12 | 0.06 | 0.12 |
| 7-MDCA | 3.43 | 405.3 | 405.3 | 0.265±0.112 | 2.7288±2.1263* | 0.5026±0.2601* | 0.7531±0.5404## | 0.579±0.4514 | 10.30 | 1.90 | 2.84 | 2.18 |
| GCA | 3.45 | 464.3 | 74 | 0.0051±0.0021 | 0.0009±0.0005*** | 0.001±0.0011*** | 0.0012±0.0009 | 0.0011±0.0006 | 0.18 | 0.20 | 0.24 | 0.22 |
| TCDCA | 3.97 | 498.3 | 80 | 0.197±0.1831 | 1.5298±1.2291* | 1.1785±0.6616** | 0.8986±0.681 | 0.8904±0.4683 | 7.76 | 5.98 | 4.56 | 4.52 |
| UDCA | 4.05 | 391 | 391 | 0.0406±0.0118 | 13.22±4.331*** | 10.8291±3.3802*** | 8.4961±4.6281# | 9.9859±4.6574 | 325.56 | 266.68 | 209.23 | 245.91 |
| GCDCA | 4.13 | 448.3 | 74 | 0.0057±0.0022 | 0.0052±0.0017 | 0.0059±0.0013 | 0.0057±0.0023 | 0.0076±0.0039 | 0.90 | 1.03 | 1.00 | 1.33 |
| TDCA | 4.15 | 498.3 | 80 | 0.5944±0.1754 | 0.0408±0.0419*** | 0.0067±0.0117*** | 0.0583±0.0866 | 0.0042±0.0053 | 0.07 | 0.01 | 0.10 | 0.01 |
| HDCA | 4.37 | 391 | 391 | 0.0997±0.0883 | 1.1284±0.6062** | 0.4921±0.2867** | 0.3741±0.2657## | 0.4804±0.2867 | 11.32 | 4.94 | 3.75 | 4.82 |
| GDCA | 4.37 | 448.3 | 74 | 0.0013±0.0009 | 0.0009±0.0005 | 0.001±0.0005 | 0.0009±0.0004 | 0.0009±0.0007 | 0.69 | 0.79 | 0.66 | 0.68 |
| CA | 4.61 | 407.3 | 407.3 | 2.5382±1.0373 | 0.7663±0.66** | 0.4553±0.5234*** | 0.4928±0.4746 | 0.9349±0.8208 | 0.30 | 0.18 | 0.19 | 0.37 |
| CDCA | 5.73 | 391 | 391 | 0.0502±0.0222 | 1.6084±1.1658** | 1.0426±0.6207** | 0.481±0.3377## | 0.9791±0.5268& | 32.06 | 20.78 | 9.59 | 19.52 |
| DCA | 5.92 | 391 | 391 | 0.8958±0.3654 | 0.9238±0.591 | 0.5648±0.2739* | 0.4138±0.2587# | 0.6737±0.6199 | 1.03 | 0.63 | 0.46 | 0.75 |
| LCA | 7.03 | 375.3 | 375.3 | 0.0956±0.0412 | 0.6162±0.1859*** | 0.1849±0.3937 | 1.1976±0.7897 | 0.0897±0.0624& | 6.45 | 1.93 | 12.53 | 0.94 |
*
P<0.05,
**
P<0.01,
***
P<0.001 vs Con group;
#
P<0.05,
##
P<0.01,
###
P<0.001 vs HFD group;
&
P<0.05 vs SV group.
Comparisons between groups were analyzed with two-tailed Student’s t-test. Con: control group; HFD: high-fat/cholesterol diet group; AB: antibiotic group; SV: simvastatin (20 mg/kg) group; AB_SV: simvastatin (20 mg/kg) in the presence of antibiotic treatment.