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. 2017 Jul 8;8(51):88259–88275. doi: 10.18632/oncotarget.19111

Figure 3. Skl regulates FGH expression.

Figure 3

A., Western blotting analysis of FGH protein expression in HEK293 cells following overexpression of Skl. FGH protein expression was normalized to β-actin. B., Quantification of FGH protein expression in HEK293 cells following overexpression of Skl. (*p < 0.05 vs. control, N = 3 independent experiments). C., Real-time RT-PCR analysis of FGH mRNA expression in HEK293 cells following overexpression of Skl. (*p < 0.05 vs. control, N = 3). D., Western blot analysis of Skl protein expression in H295R cells following silencing of Skl. E., Western blot analysis of FGH protein expression in H295R cells following silencing of Skl. F., Quantification of FGH protein expression in H295R cells following silencing of Skl. (*p < 0.05 vs. control). G., Real-time RT-PCR analysis of FGH mRNA expression following silencing of Skl. (***p < 0.001 vs. control, N = 3). Bars, means ± SEM.