Figure 1. Pim-1 and PCNA expression in the tunica media (M) of the aortic arches, thoracic and abdominal arteries of STZ-induced hyperglycemia (HG) and normoglycemia (NG) rats.

A. Representative photographs of Pim-1 and PCNA protein expression in rat aortic arches, thoracic and abdominal arteries. Immunohistochemical analysis was used to detect Pim-1 and PCNA protein expression in the tunica media of arteries. Antigens were localized by DAB and tissues were counterstained with hematoxylin. Positive rates for Pim-1 and PCNA expression were calculated using at least three representative visions (200×) for each arterial sample. Both Pim-1 and PCNA were localized in the cytoplasm and nucleus. B. Non-immune IgG was used as a negative control. Original magnification: 200×, Bar = 100 μm. C. Pim-1 and PCNA mRNA expression in the tunica media of thoracic arteries were examined by qRT-PCR. NG, Normoglycemia (n = 7); HG, Hyperglycemia (n = 10).