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. 2017 Jul 18;8(51):88320–88331. doi: 10.18632/oncotarget.19368

Figure 3. Quercetagetin and stattic attenuate HG-induced VSMC proliferation in vitro.

Figure 3

A. Cells (2 × 103) were plated into 96-well plates and 6 hours later medium was changed to either NG (5.5 mmol/L D-glucose), Mtol (25 mmol/L D-mannitol), HG (25 mmol/L D-Glucose), HG+Quer (25 mmol/L D-Glucose + 5.5 μmmol/L quercetagetin), HG+Stattic (25 mmol/L D-Glucose + 10 μmol/L stattic), or HG+DMSO (25 mmol/L D-Glucose + 0.3% (v/v) DMSO) for 48 hours and the CCK-8 assay was used to detect cell viability. **P < 0.01 vs. NG or Mtol, #P < 0.05 vs. HG or HG+DMSO, ##P < 0.01 vs. HG or HG+DMSO. B. Cells (5×105) were plated into 6-well plates and 6 hours later the medium was changed as described above for an additional 48 hours and cell numbers were counted. *P < 0.05 vs. NG or Mtol; #P < 0.05 vs. HG or HG+DMSO. C. qRT-PCR and D. western blotting were used to examine the expression of PCNA. **P < 0.01 vs. NG or Mtol; #P < 0.05 vs. HG or HG+DMSO, ##P < 0.01 vs. HG or HG+DMSO. Quer, quercetagetin.