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. 2017 Aug 3;8(51):88332–88344. doi: 10.18632/oncotarget.19917

Figure 1. Valproate hinders podocyte acquisition of de novo immune phenotypes following LPS exposure.

Figure 1

Immortalized murine podocytes were cultured and differentiated under nonpermissive conditions. Cells were injured with LPS (100µg/ml) or vehicle for 24 h in the presence or absence of sodium valproate (VPA; 0.5 or 1.5 mM). A. Cell lysates or conditioned media were collected and prepared for immunoblotting for indicated proteins. B. Immunoblots were subjected to densitometric analysis and arbitrary units were expressed respectively as immunoblot densitometric ratios of diverse molecules to actin as folds of a designated group. *P < 0.05 versus the same molecule in other groups; #P < 0.05 versus CCL5 levels in other groups (n = 3). CTSL, cathepsin L; LPS, lipopolysaccharide; VPA, sodium valproate.