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. 2017 Aug 3;8(51):88332–88344. doi: 10.18632/oncotarget.19917

Figure 3. The LPS-elicited GSK3β overactivity and NFkB hyperactivation in podocytes is obliterated by valproate.

Figure 3

Podocytes were treated as elaborated in Figure 1. A. Cell lysates were collected and prepared for immunoblotting for phosphorylated GSK3β at serine 9 residue, phosphorylated NFkB RelA/p65 at serine 467 residue and other indicated proteins. B. Immunoblots were subjected to densitometric analysis and arbitrary units of p-GSK3β and p-RelA were expressed respectively as immunoblot densitometric ratios of p-GSK3β/GSK3β and p-RelA/p65/RelA/p65 as folds of the control group. Linear regression analysis indicated a statistically significant (P < 0.05) inverse correlations between p-GSK3β and p-RelA/p65 in podocytes. LPS, lipopolysaccharide; VPA, sodium valproate.