Figure 2. TgESAs induce Ana-1 cell apoptosis.

Ana-1 cells were treated for 48 h with different concentrations (0, 5, 10, 20, 40, 80 μg/mL) of TgESAs. (A) The flow cytometry data showed one representative dual staining result. (B) Early- stage (%) and late-stage (%) apoptotic cell death were detected by staining cells with Annexin V-FITC and PI, and analyzing by flow cytometry. *P < 0.05 and **P < 0.01 compared with untreated group (0 μg/ml).