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. 2016 Aug 19;8(51):88386–88400. doi: 10.18632/oncotarget.11393

Figure 1. SH003 induces apoptotic cell death.

Figure 1

(A) Cell viability was measured by MTT assay. MDA-MB-231 and HCC-38 cells were seed in 96-well plates and treated with various concentration of SH003 (0, 100, 250 and 500 μg/ml) for 48 hours. Data were analyzed by ANOVA with P < 0.05. (B) After MDA-MB-231 cells were treated with 500 μg/ml of SH003 for 48 hours, live and dead assay was done by using live and dead cell assay kit. Dead cells (Red fluorescence-positive cells) were counted. *P < 0.05. (C) MDA-MB-231 and HCC-38 cells were treated with different doses of SH003 for 48 hours. Cells were stained with Annexin V and 7AAD at room temperature in the dark. Annexin V-positive apoptotic cells were detected using FACSCalibur. *P < 0.05. Graph shows annexin V-positive apoptotic cells (%) calculated from the total amount of right-upper and -lower portion. (D) Cells were treated with 500 μg/ml of SH003 for 24 hours and then performed western blots with anti-Bax, -Bcl2, -Cleaved caspase 3 and PARP. Actin was used for the internal control. Experiments were performed in triplicate. Bars indicate means that standard deviations (SD).