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(A) Tracer auto-phagosome formation. The images show the results of transfection with pcDNA and pcDNA-GFP–LC3, NC-siRNA and pcDNA-GFP–LC3, pcDNA-AM and pcDNA-GFP–LC3, and siRNA-839 and pcDNA-GFP-LC3 vectors, respectively. (B) Western blots showing the LC3-II/I ratio and Beclin levels were not changed. (C) Gray-scale scanning histogram of (B). The data represent the mean ± SEM (P-value=0.0120; P-value=0.0071).
