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. 2017 Aug 3;8(51):88537–88551. doi: 10.18632/oncotarget.19877

Figure 3. Adiponectin inhibits proliferation of prostatic epithelial and stromal cells.

Figure 3

(A, B) AdipoR1 knockdown efficiency was confirmed using western blotting. (C, D) CCK-8 proliferation analysis of RWPE1 and WPMY1 cells that were transfected with lentiviral vectors containing AdipoR1-shRNA or a scrambled sequence as a control. Cells were cultured in basic medium with or without 10ng/ml of EGF and then treated with 0, 1, 5, 10 or 20 μg/ml human recombinant adiponectin. The results are expressed as the mean±s.d. of three independent experiments (one-way analysis of variance followed by Dunnett post-tests; ***p<0.001, ns, not significant).