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. 2017 Aug 9;8(51):88599–88612. doi: 10.18632/oncotarget.20100

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Copyright: © 2017 Zhu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Disruption of the β-TrCP2, not β-TrCP1 gene, induces accumulation of ZNF281 in MEF cells (A) Measurement of ZNF281 protein level in MEFs of the indicated β-TrCP2 and β-TrCP1 genotypes. Tubulin served as a loading control. (B) Measurement of ZNF281 mRNA levle in MEFs of the indicated β-TrCP2 and β-TrCP1 genotypes using RT-qPCR. (C) Measurement of ZNF281 in MEFs of the indicated genotypes treated with CHX (25 μg/ml) for the indicated times. And relative ZNF281 abundance normalized by tubulin abundance.