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. 2017 Sep 12;8(51):88719–88729. doi: 10.18632/oncotarget.20838

Figure 1. STAT3 is activated in a subgroup of esophageal squamous cell cancers.

Figure 1

(A) Expression of STAT3 and pSTAT3 in primary ESCC samples from 8 patients were examined by immunoblotting. (B) Expression of STAT3 and pSTAT3 in 8 different lines of ESCC cells were examined by immunoblotting. (C) EC9706 cells were treated with indicated doses of cytokines and growth factors for 30 minutes. They were harvest to assess the expression of STAT3 and p-STAT3. (D) and (E) The proliferation of EC9706 and TE13 were examined by using CCK-8 after treated with 10 ng/ml of IL-6, LIF, EGF, or INF-γ for 48h. Cells cultured with medium alone were regarded as 100%. (F) After treated with indicated doses of 5-FU, the growth of EC9706 and TE13 cells were examined by using CCK-8.