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. 2017 Oct 4;8(51):88845–88856. doi: 10.18632/oncotarget.21483

Figure 5. Serial dilution of notochordal cell-conditioned medium (NCCM), and NCCM-derived extracellular vesicles (EVs) and proteins (P).

Figure 5

(a) DNA content, (b) GAG content, (c) GAG/DNA content (mean ± SD) of canine CLC micro-aggregates cultured for 7 days in control culture medium, unfractionated porcine NCCM, or porcine NCCM-derived EVs or proteins. EVUC: NCCM-derived EVs obtained after size exclusion chromatography (SEC) and subsequent 100,000g ultracentrifugation (UC), PUC: NCCM-derived proteins obtained after SEC and subsequent 100,000g UC. Grey line represents controls (set at 100%). Black bars indicate significant differences between conditions (p<0.05); *, **,***: significantly different from controls (p<0.05, p<0.01, p<0.001, respectively). n = 6 porcine NCCM, EV, and P donors tested on a pool of 4 canine CLC donors. GAG: glycosaminoglycan. n.s.: not significantly different.