Skip to main content
. 2017 Oct 4;8(51):88988–89004. doi: 10.18632/oncotarget.21640

Figure 8. Knockdown of CK2α decreased nuclear translocation, transcriptional activity and DNA binding activity of NF-κB in SphK1-overexpressed GMCs.

Figure 8

(A and C) Data of western blotting and immunofluorescence showed that knockdown of SphK1 caused no evident change on nuclear translocation of NF-κB p65 in CK2α-overexpressed GMCs. Values represent means ± S.E. (n = 3 independent experiments. **P < 0.01 vs. Vector by one-way ANOVA). (B and D) However, nuclear NF-κB p65 was extremely downregulated and cytoplasmic NF-κB p65 was improved after CK2α knockdown in SphK1-overexpressed GMCs. Values represent means ± S.E. (n = 3 independent experiments. **P < 0.01 vs. Vector; ##P < 0.01 vs. SphK1+NC by one-way ANOVA). (E-H) Transcriptional activity and DNA binding activity of NF-κB were effectively inhibited after CK2α knockdown in SphK1-overexpressed GMCs, while knockdown of SphK1 showed no effective change on transcriptional activity and DNA binding activity of NF-κB in CK2α-overexpressed GMCs. Values represent means ± S.E. (n = 3 independent experiments. **P < 0.01 vs. Vector; #P < 0.05 vs. SphK1+NC by one-way ANOVA).