Fig 5. Analysis and parameter estimation from identified colonies.

(A) kymograph of the pixel signal in the central slice of an example colony. The x marks show the radius estimated for each time and the red line is the radius value obtained from a fitted area function (also red circles). (B) Colony area curve fitting for some example colonies. Dots are the estimated area from the standard deviation of the blobs and the continuous lines are the model fitting of this values (C) Mean fluorescence intensity signal over time for the example colonies. Lines were obtained by spline smoothing. (D) Colony growth rate estimation from the data for each selected colony. (E) Protein expression rate parameter estimation using the previous data.