Fig 2. Typical images of the follow-up of a SW480 MCTS.

Images taken at 0h, 24h, 32h and 48h in the absence of drug (A-F) or in presence of 10 μM of 5-FU dispensed at initial time 0h (G-L). The dotted line representing the initial spheroid size in A, G is reported on other images as a guide for the eye. Curved arrows indicate the spheroids transfer into a new well. Straight short arrows show the diffuse cell outer layer. Scale bar: 200 μm.