Table 1. IC₅₀ values for 5-FU for the three cell lines at 24h and 48h in 2D versus 3D spheroids.

A range of IC₅₀ values (e.g., 1–2 μM) indicates that the precise IC₅₀ was in between two concentrations tested.

		24h			48h
		SW480	HCT116	HT29	SW480	HCT116	HT29
IC50	2D	1-2 μM	50-100 μM	> 100 μM	< 1 μM	2-5 μM	100 μM
	3D	> 300 μM	> 300 μM	> 300 μM	1-5 μM	1-5 μM	> 300 μM