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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Mol Psychiatry. 2016 Oct 11;22(10):1483–1491. doi: 10.1038/mp.2016.157

Figure 2.

Figure 2

Cre-recombinase expressed under control of the parvalbumin promoter in PV-Cre mice manifests in a developmentally late pattern in both pre- and postsynaptic neurons of the calyx of Held synapse. (a) Low-magnification image of a single focal plane of a section of the brainstem and cerebellum from PV-Cre+ mice crossed with tdTomato reporter mice at P12 (red = tdTomato fluorescence; blue = DAPI staining). Note that tdTomato is robustly expressed in the ventral cochlear nucleus (VCN) and medial nucleus of the trapezoid body (MNTB). (b) High-magnification images of an MNTB section from a PV-Cre/tdTomato reporter mouse at P12, viewed in a single focal plane (red = tdTomato fluorescence; green = immunolabeling for synaptotagmin-2 (Syt2)). The white arrow depicts the position of the fluorescence intensity plot depicted below the images. (c) Analyses of Cre-expression in calyx synapses of PV-Cre mice as a function of developmental time analyzed in PV-Cre/tdTomato reporter mice. The age of the mice examined is listed above the panels. P, postnatal day.