Skip to main content
. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Mol Psychiatry. 2016 Oct 11;22(10):1483–1491. doi: 10.1038/mp.2016.157

Figure 3.

Figure 3

Developmentally late, but not developmentally early conditional NL3 KO reduces AMPAR-mediated synaptic transmission at the calyx of Held. (a) Cartoon of PV-Cre activity during development of MNTB. PV-20-Cre activity starts at early postnatal stages. Crossing PV-Cre mice with NL3-cKO mice will results in early postnatal NL3 deletion. (b–d), Sample traces and summary data of EPSCs (b), and EPSCs train (c, d) recorded from P11-13 MNTB neurons at control (black) or PV-NL3 (red) littermate. (e) Cartoon of Krox-20-Cre activity in development of MNTB. Krox-20-Cre activity starts at early embryonic stages, even before axons of calyx of Held cross the midline. Crossing Krox-20-Cre mice with NL3-cKO mice will results in early prenatal NL3 deletion. (f–h), Same as (b–d), but for P11-13 control and Krox-20-NL3 littermate. Data are mean ± s.e.m. Numbers in bars represent the numbers of cells/animals. Statistical significance was determined by two-tailed Student's t-test or by single-factor analysis of variance (ANOVA) (*P < 0.05; **P < 0.01; ***P < 0.001; non-significant comparisons are not labeled). AMPAR, AMPA receptor; EPSC, excitatory postsynaptic currents; P, postnatal day; MNTB, medial nucleus of the trapezoid body.