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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Cell Rep. 2017 Oct 24;21(4):926–933. doi: 10.1016/j.celrep.2017.09.088

Fig. 4. Over-expression of the KCNT2 Phe240Leu mutation causes membrane hyperexcitability in primary neurons.

Fig. 4

Representative action potential recordings of (A) un-transfected, (B) empty pTRACER-, (C) WT rSlick- and (D) F240L rSlick-transfected neurons. Equal amounts (0.5 μg) of wild-type rSlick or Phe240Leu rSlick or empty pTRACER vector were transfected into embryonic rat DRG neurons plated on 18 mm glass coverslips. Whole cell current clamp was performed 24–48h post-transfection. Current-clamp recordings were performed using an injection of a supra threshold stimulus of 400 pA for 1000ms. Shown are individual traces for a total n=6 for the un-transfected, pTRACER-, WT rSlick- and F240L rSlick-transfected groups.