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. Author manuscript; available in PMC: 2018 Oct 25.
Published in final edited form as: Cell Syst. 2017 Oct 18;5(4):410–417.e4. doi: 10.1016/j.cels.2017.09.012

Figure 2. Faster oscillations of p53 in mouse cells are not due to the p53 sequence, general metabolic state or DNA damage signaling.

Figure 2

(A) Human (MCF7) or Mouse (NIH3T3) cells expressing either human or mouse p53-YFP were DNA damaged (NCS 100ng/ml) and imaged for 15hrs (faint lines indicate single cells, bold lines indicate the average). Oscillations of p53 were quantified and the period was calculated with autocorrelation function (N>20 cells). (B) Human (MCF7) or Mouse (NIH3T3) cells expressing RELA-CFP were treated with TNFα (10ng/ml) and imaged for 6hrs. RelA-CFP nuclear signal was quantified and autocorrelations was used to calculate the oscillatory period (N>20 cells). (C) Human (MCF7) or Mouse (HEPA1c1c7) cells expressing a dox inducible p53-YFP construct were imaged after addition of doxycycline (50ng/ml). Three examples traces are shown and the quantified periods (N>10 cells).