Figure 7. Kinetics of nitrogenase activity in N₂-fixing M. pudica nodules.

Plants were inoculated with C. taiwanensis CBM832 (Fix⁺), and nitrogenase activity measured using the acetylene reduction assay (ARA) (Figure 7—source data 1). Two negative controls, i.e. tubes containing only the acetylene substrate and plants inoculated with C. taiwanensis CBM2568 (Fix^-), were included. In these cases, boxplots correspond to data from all time points. *, Significantly different from the negative controls (p<0.05 after multiple comparison test of Kruskal-Wallis).