Table 2. Strains and plasmids used in this study.
| Bacterium | Strain | Relevant characteristics | Reference/source |
|---|---|---|---|
| E. coli | DH5α | F recA lacZM15 | Bethesda research laboratory |
| DH5α λpir | F recA lacZM15 λpir | HP Schweizer | |
| C. taiwanensis | LMG19424 | Wild-type strain isolated from Mimosa pudica in Taiwan | (Chen et al., 2001) |
| CBM832 | LMG19424 derivative resistant to Streptomycin, StrR | M. Hynes | |
| CBM2568 | CBM832 deleted in nifH, StrR | This study | |
| CBM2700 | CBM832 carrying a Pps-GFP fusion downstream glmS, StrR | This study | |
| CBM2701 | CBM2568 carrying a Pps-GFP fusion downstream glmS, StrR | This study | |
| CBM2707 | CBM2568 carrying a Pps-mCherry fusion downstream glmS, StrR | This study | |
| CBM2708 | CBM832 carrying a Pps-mCherry fusion downstream glmS, StrR | This study | |
| Plasmids | Name | Relevant characteristics | Reference/source |
| pGPI-SceI | oriR6K, mob+, carries a I-SceI site, TriR | (Flannagan et al., 2008) | |
| pDAI-SceI | oripBBR1, mob+, carries the I-SceI gene, TetR | (Flannagan et al., 2008) | |
| pRCK-Pps-GFP | Plasmid carrying the psbA promoter region fused to GFP, KanR | M. Valls | |
| pRCK-Pps-mCherry | Plasmid carrying the psbA promoter region fused to mCherry, KanR | M. Valls | |
| pCBM156 | pGPI-SceI carrying the nifH 5’ and 3’ regions, TriR | This study | |
| pCBM161 | pGPI-SceI carrying the glmS-Ralta_A0206 intergenic region interrupted by a Pps-GFP fusion, TriR | This study | |
| pCBM162 | pGPI-SceI carrying the glmS-Ralta_A0206 intergenic region interrupted by a Pps-mCherry fusion, TriR | This study | |
| pRK2013 | Helper plasmid, KanR | (Figurski and Helinski, 1979) |
Str, spreptomycin; Tri, trimethoprim; Tet, tetracycline; Kan, kanamycin.