(A–U) Transverse sections through the cochlear duct of control
(n=15), Esrp1−/−
(n=7) and
Esrp1−/−;Fgf9+/−
(n=6) embryos at E18.5 immunostained for E-cadherin (A,H,O), Otx2
(B,I,P) and cell type specific markers of the stria vascularis (C–G,
J–N, Q–U). Scale bars = 50μm. (V) Quantification
of cells expressing Otx2 and Nr3b2 represented as mean ± SD
(***P<0.0001, ANOVA with Tukey’s test). (W)
Schematic representation of the lateral cochlear wall phenotypes manifesting
from altered Fgf signaling in
Fgf10−/− and
Esrp1−/− mutants compared to
a control embryo. See also Figure S6.