FIG 3 .

dNSP16 mutant MERS-CoV is attenuated in primary cultures and in vivo. (A) Primary HAE cells infected with WT (black) or dNSP16 mutant (red) MERS-CoV at an MOI of 0.01 and monitored over a time course. (B) Day 2 and 4 lung titers of adenovirus-transduced mice expressing human Dpp4 infected with WT (black) or dNSP16 mutant (red) MERS-CoV. (C) Day 2 and 4 lung titers of 288-330^+/+ CRISPR-Cas9-targeted mice infected with WT (black) or dNSP16 mutant (red) MERS-CoV. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (Student t test). n = ≥4 for each experimental group at each time point over multiple experiments.