FIG 5 .
dNSP16 mutant MERS-CoV protects from a lethal challenge. Graphs show weight losses (A), day 4 viral titers (B), and hemorrhage scores (C) following a challenge of 288-330+/+ CRISPR-Cas9-targeted mice vaccinated with dNSP16 mutant MERS-CoV (red) or mock vaccinated (black) with 106 PFU of passaged mouse-adapted MERS-CoV (15). (D) Plaque reduction neutralization with serum from WT (black) or dNSP16 mutant (red) MERS-CoV-vaccinated mice. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Student t test). n = ≥4 for each experimental group at each time point in multiple experiments.