FIG 3 .

Depletion of SNF6 led to multiple phenotypes relevant to C. albicans fitness and pathogenicity. (A) WT and snf6/Tet-SNF6 cells were serially diluted and grown on plates under nonrepressing (YPD) or standard repressing (YPD plus 20 µg/ml doxycycline) conditions for 3 days at 30°C, and the resulting colonies were photographed. Cells from snf6 mutant colonies were observed by microscopy using differential interference contrast for bright field or through the 4′,6-diamidino-2-phenylindole (DAPI) filter for calcofluor white staining. (B) WT and snf6/Tet-SNF6 cells were grown in different solid media with different carbon sources under both repressing (40 µg/ml doxycycline) and nonrepressing conditions. Pictures were taken after 3 days of growth. WT refers to strain CAI4. (C) Snf6 is required for hyphal development. WT and snf6/Tet-SNF6 cells were grown in YPD plus 10% FBS and Spider and M199 media to promote invasive hypha formation in the presence or the absence of doxycycline. Representative pictures were taken after 3 days of growth.