Table 1. Primers and PCR conditions used to amplify genomic DNA segments of the SHOX gene.

Primer	Forward primer (5′-3′)	Reverse primer (5′-3′)	Annealing temperature (℃)/time	Product size (bp)
SX-1	GGAGCCAATAGGGGTCTTCG	GCTTGGTGAGCTCGGTTTTG	55/30 sec	308
SX-2	CTCTCTCCAGCCGTGAACTC	CGAGAGTGGCAGAAGGTAA	55/45 sec	758
SX-3	CACGTTGCGCAAAACCTCC	GGAGCCATCGCCTGGTC	55/30 sec	312
SX-4+5	TTCACAGGGCTCTTCACATC	CTTCCCCTTTCCCCTATTTGC	55/30 sec	376
SX-6a-1	CCCCCAGTCCCCATCCTG	CCTCCCAGGCAGCAATAAGG	62/50 sec	871
SX-6a-2	CCTCCCAGGCAGCAATAAGG	CATCTTCTGCGTTCCCCCAG	55/45 sec	696
SX-6a-3	CTCCATCTTCTGCGTTCCCCCAG	TGGGAAGTAGGTTCATTCATCAG	55/1 min	1,034
SX-6b	CAGCCTCCCAAAGTGCTGGGACTAC	TGTACAGTTTGAGGGTGTGTGTGC	60/45 sec	752