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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Laryngoscope. 2017 Jul 17;127(12):2713–2720. doi: 10.1002/lary.26737

Figure 2.

Figure 2

Figure 2

Five C57BL/6 mice were vaccinated twice weekly with 2μg CRT/HPV11-E6E7 DNA vaccine via electroporation, and splenocytes were harvested one week after the final vaccination. Splenocytes were stimulated with 1μg/ml overlapping HPV11 E6 peptides (A and B) or HPV11 E7 peptides (C and D) in the presence of 1μg/ml GolgiPlug. Cells were stained with PE anti-mouse CD8a and FITC anti-mouse IFN-γ and analyzed by flow cytometry. Sample flow cytometry data from an individual mouse gated for 0.5 × 105 cells, bar graph presented for 3 × 105 cells to normalize with previously published literature.