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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Laryngoscope. 2017 Jul 17;127(12):2713–2720. doi: 10.1002/lary.26737

Figure 3.

Figure 3

C57BL/6 mice were vaccinated either once a week or 3 consecutive days a week for 3 weeks with 2μg CRT/HPV11 E6E7 DNA via electroporation. Unvaccinated mice were used as a control. Splenocytes were harvested one week after the final vaccination. Splenocytes were stimulated with 1μg/ml HPV11-E6aa41-70 peptide in the presence of 1μg/ml GolgiPlug. Cells were stained with PE anti-mouse CD8a and FITC anti-mouse IFN-γ and analyzed by flow cytometry. Sample flow cytometry data from an individual mouse gated for 3 × 105 cells.