Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
C57BL/6, BALB/c and SKH1 mice were vaccinated twice weekly with 2μg CRT/HPV11-E6E7 DNA vaccine via electroporation, and splenocytes were harvested one week after the final vaccination. Splenocytes were stimulated with 1μg/ml overlapping A) HPV11 E6 peptides or B) HPV11 E7 peptides in the presence of 1μg/ml GolgiPlug. 3 × 105 cells were stained with PE anti-mouse CD8a and FITC anti-mouse IFN-γ and analyzed by flow cytometry.
